Anti-IL-10R [1B1.3a](MABL-3454)

Cat. No.

MABL-3454

Name

Anti-IL-10R [1B1.3a](MABL-3454)

Clone No.

1B1.3a

From

Recombinant Antibody

Isotype

Engineer antibody

Application

Blocking, FC

Species Reactivity

Mouse

Basic Information

Specificity

This antibody recognises mouse IL-10R which   is a cell surface glycoprotein and a receprot for anti-inflammatory IL-10.   The epitope recognised by this antibody has been localised to the ligand   binding domain within the extracellular region of mouse IL-10R.

Alternative Name

CD210; Interleukin-10 receptor subunit; Interleukin-10 receptor subunit alpha; Il10ra Il10r; IL-10 receptor subunit alpha; IL-10R subunit alpha; IL-10RA; CDw210a; Interleukin-10 receptor subunit 1; IL-10R subunit 1; IL-10R1; IBI-3A; 1B1-3A

UniProt

Q61727

Immunogen

This antibody was raised by immunising rats   with the purified recombinant extracellular region of mouse Interleukin 10   Receptor.

Application Notes

1B1.3a has been widely applied in flow cytometric analyses where the detection of IL- 10R was needed. Furthermore, this antibody was also successfully utilised as a blocking agent; one group used   it to demonstrate that IL-10 mediates suppressive activity, by targeting its receptor IL-10R with this antibody (Wang et al., 2004). Castro et al. (2000)   demonstrated that 1B1.3a is able to make a soluble peptide or protein antigen   immunogenic for subsequent Th1 recall responses in a situation when there is   an additional signal, such as LPS, activating the immune system.   Consequently, this antibody is a hugely promising agent in the potential   development of vaccines for immunotherapy. Similarly, Vicari and colleagues   (2002) showed that this antibody has an enormous potential as a therapeutic   anti-tumour agent when combined with the administration of CpG to stimulate   the immune system.

Antibody First   Published

O'Farrell et al. IL-10 inhibits macrophage activation and proliferation by distinct signaling mechanisms: evidence for   Stat3-dependent and -independent pathways. EMBO J. 1998 Feb 16;17(4):1006-18.   PMID:9463379

Note on publication

This article describes the method of generation of antibodies like 1B1.3a.

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified   antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity   purified

Buffer

PBS with 0.02% Proclin   300.

Concentration

1 mg/ml.

Storage Recommendation

Store at 4⁰C for up to 3   months. For longer storage, aliquot and store at - 20⁰C.