Anti-Lag3 [C9B7W](MABL-3452)

Cat. No.

MABL-3452

Name

Anti-Lag3 [C9B7W](MABL-3452)

Clone No.

C9B7W

From

Recombinant Antibody

Isotype

Engineer antibody

Application

Blocking, IP, FC

Species Reactivity

Mouse

Basic Information

Specificity

This antibody is specific for an epitope in the D2 domain of murine LAG-3.

Alternative Name

CD223; LAG3; Lymphocyte activation gene 3 protein

UniProt

Q61790

Immunogen

This antibody was raised by immunising Lewis   rats with a murine LAG-3:Ig fusion protein.

Application Notes

This antibody has been used in flow cytometric analysis of splenocytes (Workman et al, 2005), T cell hybridomas   expressing wild‐type or chimeric CD4:murine LAG-3 (Workman et al, 2002) and CD4+ regulatory T cells (Chien et   al, 2017). It has also been used in immunoprecipitation analysis of murine T   hybridoma 3A9 cells transduced with murine LAG-3 (Li et al, 2004). This   antibody blocks LAG-3 function in vitro, inhibiting antigen‐induced IL‐2 production in LAG-3-expressing T cell hybridomas, but does not block LAG-3:MHC class II interaction (Workman et al, 2002).   Treatment with this antibody also blocks LAG-3 function in vivo, leading to accelerated homeostatic expansion of WT T cells (Workman et al, 2005). Administration of this LAG-3-blocking antibody, in combination with PD-L1 blockade, synergistically improves the survival of lymphodepleted   myeloma-bearing mice (Jing et al, 2015).

Antibody First   Published

Workman et al. Phenotypic analysis of the murine CD4‐related glycoprotein, CD223 (LAG‐3) Eur J Immunol. 2002 Aug;32(8):2255-63 PMID:12209638

Note on publication

Describes the original generation of this antibody, and its characterisation.

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified   antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity   purified

Buffer

PBS with 0.02% Proclin   300.

Concentration

1 mg/ml.

Storage   Recommendation

Store at 4⁰C for up to 3   months. For longer storage, aliquot and store at - 20⁰C.