Key features and details | |
Cat. No. | MABL-3594 |
Name | Anti-Spike protein mabs |
Clone No. | EY6A |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | NTRL, SurfacePlasmonResonance, therapeutic, ELISA, IF |
Species Reactivity | SARSCoronavirus2 (SARS-Cov-2) |
Basic Information | |
Specificity | This antibody binds tightly (KD of 2 nM) to the receptor binding domain (RBD) the of the SARS- CoV-2. It also cross reacts with SARS-CoV-1. This antibody recognizes a highly conserved epitope on away from the ACE2 receptor binding domain. |
Alternative Name | SARS CoV 2 S glycoprotein; COVID-19 Spike protein; RBD; Receptor Binding Domain; E2 glycoprotein; E2; Human coronavirus 2 spike glycoprotein; Peplomer protein; S glycoprotein; SARS coronavirus 2 S protein; SARS coronavirus 2 Spike Protein; SARS CoV 2 Spike protein; SARS CoV 2; SARS-CoV-2 S protein; SARSCoV2; SARS-COV-2 S protein; SARS-COV-2 Spike glycoprotein; SARSCOV2 Spike protein; Severe acute respiratory syndrome 2 spike glycoprotein; Severe acute respiratory syndrome virus 2 spike glycoprotein; Spike glycoprotein; 2019-nCoV |
UniProt | P0DTC2 |
Immunogen | The original antibody was isolated by cloning antibody genes from blood derived plasmablasts of a Covid-19 infected patient in the convalescent phase. |
Application Notes | EY6A binds S1 domain of SARS-CoV-2 and also cross reacts with SARS-CoV-1. The binding was confirmed by ELISA. Further confirmation of binding of EY6A to SARS-CoV-2 infected cells was done by immunofluorescence. Surface plasmon resonance (SPR) measurements for EY6A Fab showed high affinity binding to immobilised SARS-CoV-2 RBD (KD = 2 nM). EY6A did not completely block binding of ACE2 to RBD, but was successful in neutralizing SARS-CoV-2 infection in Vero E6 cells (Zhou et al., 2020). |
Antibody First Published | Zhou et al. Structural basis for the neutralization of SARS-CoV-2 by an antibody from a convalescent patient. BioRxiv (2020) |
Note on publication | Describes the generation, characterization and structural complex formed by this antibody with the receptor binding domain of the SARS-CoV-2. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
