Key features and details | |
Cat. No. | MABL-3550 |
Name | Anti-Spike Protein (RBD) |
Clone No. | CV30 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | neutralize, therapeutic, ELISA, FC |
Species Reactivity | SARSCoronavirus2 |
Basic Information | |
Specificity | This antibody recognizes and binds the receptor binding domain (RBD) encoded by residues 319-591 of the SARS CoV 2 spike protein. CV30 binds the SARS CoV 2 RBD with a high affinity of 3.6nM and completely blocks the interaction between the RBD and ACE2 domain. |
Alternative Name | Receptor Binding Domain; SARS-CoV2; SARS Coronavirus 2; SARS-CoV-2; SARS CoV 2; 2019-nCoV; SARS Coronavirus 2 receptor binding domain; SARS CoV2 RBD; Severe acute respiratory syndrome coronavirus 2; Spike protein; S protein; SARS-CoV 2 S protein; S glycoprotein; E2; Peplomer protein |
UniProt | P0DTC2 |
Immunogen | The original antibody was generated by isolating B-cells from the peripheral blood mononuclear cells (PBMCs) of SARS CoV2 infected patients, after the serum neutralizing activity was confirmed. |
Application Notes | This antibody is a potent 2019-nCoV neutralizing antibody and highly recommended for diagnosis and/or treatment of SARS CoV 2 or 2019-nCoV. CV30 binds SARS CoV 2 RBD with a very high affinity and achieves 100% neutralization (IC50=0.03µg/ml). ELISA was used to confirm the binding of SARS CoV 2 positive serum samples to the entire ectodomain and the receptor binding domain of the spike protein. Specific binding of the mAb to SARS CoV 2 to receptor binding domain was confirmed with biolayer interferometry. Phycoerythrin labelled mAb was used to stain 293E cells transfected with wild type SARS CoV 2 and were analysed by flow cytometry (Seydoux et al., 2020). |
Antibody First Published | Seydoux et al. Characterization of neutralizing antibodies from a SARS-CoV- 2 infected individual PMID:32511342 |
Note on publication | Describes the generation and characterization of this antibody from PBMCs of infected patients. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
