Key features and details | |
Cat. No. | MABL-633 |
Name | Anti-Cathepsin S mAbs |
Clone No. | AFD- Fsn0503 (1E4) |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | antagonist, ICC, therapeutic, WB, Block, ELISA, IF, IHC |
Species Reactivity | Human |
Basic Information | |
Specificity | This antibody binds human cathepsin S and does not cross react with cathepsin B, cathepsin L and cathepsin K. Cathepsin S is a member of the papain family of cysteine proteases and normally localized in the lysosomes of antigen presenting cells. Greater than 90% of tumors show intense expression of cathepsin S compared with weak or undetectable expression in the normal colonic epithelium. During tumor development it is secreted in the tumor microenvironment of astrocytoma, breast, colorectal and prostate carcinomas where it involved in extracellular matrix remodeling. It has been shown that it plays an important role in tumor invasion and angiogenesis. |
Alternative Name | CTSS; cysteine protease |
UniProt | P25774 |
Immunogen | The original antibody was generated by immunizing BALB/c mice with recombinant mature human cathepsin S (amino acid residues 115-331). |
Application Notes | This antibody significantly blocked invasion of a range of tumor cell lines, most significantly HCT116 colorectal carcinoma cells, through inhibition of extracellular cathepsin S–mediated proteolysis. This antibody also shows anti-angiogenic effects as it blocked endothelial cell capillary tube formation and aortic microvascular sprouting. In vivo administration of this antibody resulted in inhibition of tumor growth and neovascularization of HCT116 xenograft tumors. The binding specificity of this antibody towards cathepsin S was determined by ELISA and western blot. This antibody was also used to study the expression of cathepsin S in HCT116 cells using immunocytochemistry (PMID: 19789302). This antibody also demonstrates anti-angiogenic effects and significantly reduces microtubule formation by HUVEC cells in vitro and vessel outgrowth in the (ex vivo) rat aortic ring model (Gormley et al., AACR 101st Annual Meeting 2010, Apr 17‐21). |
Antibody First Published | Burden et al. Antibody-mediated inhibition of cathepsin S blocks colorectal tumor invasion and angiogenesis. Clin Cancer Res. 2009 Oct 1;15(19):6042-51. PMID:19789302 |
Note on publication | Describes the generation of this antibody and examines the antiangiogenic effects of this antibody. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
