Anti-Cathepsin B mAbs

Cat. No.

MABL-632

Name

Anti-Cathepsin B mAbs

Clone No.

AFD- 2A2

From

Recombinant Antibody

Isotype

Engineer antibody

Application

inhibit, WB, ELISA

Species Reactivity

Human

Basic Information

Specificity

This antibody binds human cathepsin B (Cat   B) at an epitope near the active site and inhibits its proteolytic activity.   It was reported to specifically bind the 'EPGYSP' sequence located between   amino acid residues 133–138 of cathepsin B. Cat B is a lysosomal cysteine   protease that is shown to participate in processes of tumor growth, invasion,   and metastasis. It is seen that the increased level of tumor cathepsin B is  not balanced by an increase in cysteine proteases, leading to uncontrolled   proteolysis in the extracellular matrix. Significantly increased levels of   Cat B have also been found in sera of patients with breast, colorectal,   liver, pancreatic, and melanoma cancers.

Alternative Name

Cat B; CTSB; CPSB; APPS; APP secretase;   Cathepsin B1

UniProt

P07858

Immunogen

The original antibody was generated by   immunizing BALB/c mice with highly purified recombinant human cathepsin B   expressed in E. coli.

Application Notes

 This antibody is capable of binding   cathepsin B in an antigen-immobilized ELISA. The binding reactivity of this   antibody to cathepsin B was also confirmed using Western blot. This antibody   significantly inhibited the invasion of ras-transformed MCF-10A neoT cells   through matrigel. This antibody was further reported to inhibit cathepsin B   activity against BODYPY FL casein significantly (Fan et al., 2002; PMID:   12530548). In a study, the intracellular and extracellular cathepsin B   activities in the invasion of ras- transformed human breast epithelial cells,   MCF-10A neoT, were assessed using this antibody. This antibody was shown to   impair both the intracellular and the extracellular fractions of cathepsin B   activity (Premzl et al., 2003; PMID: 12581740). 

Antibody First Published

Fan et al. Molecular cloning and   chimerisation of an inhibitory anti-cathepsin B antibody and its expression   in Chinese hamster ovary cells. Biol Chem. 2002 Nov;383(11):1817-20.   PMID:12530548

Note on publication

The original article describes the cloning   and generation of a chimeric antibody against cathepsin B in CHO cells.

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity purified

Buffer

PBS with 0.02% Proclin 300.

Concentration

1 mg/ml.

Storage Recommendation

Store at 4⁰C for up to 3 months. For   longer storage, aliquot and store at - 20⁰C.