Anti-Cardiolipin mAbs

Cat. No.

MABL-624

Name

Anti-Cardiolipin mAbs

Clone No.

AFD- A1.72

From

Recombinant Antibody

Isotype

Engineer antibody

Application

ELISA

Species Reactivity

Bovine

Basic Information

Specificity

The antibody is specific for cardiolipin.

Alternative Name

UniProt

Immunogen

The original antibody was generated by immunizing NZW x BXSB mice with bovine heart cardiolipin.

Application Notes

The specificity of the original format of   the antibody was confirmed by ELISA analysis. The dissociation constants of the original format of the antibody, recombinant scFv fragments, and the   light chain were determined by an ELISA method. The dissociation constants of   these preparations were virtually identical : 20 nM for the original format of the antibody, 18.7 nM for the scFv format, and 21 nM for the light chain   (Pereira et al., 1998; PMID: 9477972).

Antibody First Published

Pereira et al., Cardiolipin binding a light   chain from lupus-prone mice. Biochemistry. 1998 Feb 3;37(5):1430-7. doi:   10.1021/bi972277q. PMID:9477972

Note on publication

The original paper describes the generation   and characterization of the antibody.

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity purified

Buffer

PBS with 0.02% Proclin 300.

Concentration

1 mg/ml.

Storage Recommendation

Store at 4⁰C for up to 3 months. For   longer storage, aliquot and store at - 20⁰C.