Key features and details | |
Cat. No. | MABL-620 |
Name | Anti-Carcinoembryonic Antigen mAbs |
Clone No. | AFD- COL-1 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | in vivo, RIA, therapeutic, WB, IF, IHC |
Species Reactivity | Human |
Basic Information | |
Specificity | The antibody is specific for CEA. COL-1 does not cross-react with NCA, NFA-1, BGP and PMNs. CEA is one of the most commonly used tumor markers in serum immunoassay determinations of carcinoma. |
Alternative Name | CEA |
UniProt | Q13984 |
Immunogen | The original antibody was generated by immunizing BALB/c mice with extracts or membrane- enriched fractions of biopsy material from either primary or metastatic colon carcinoma lesions. |
Application Notes | The antibody reacted with purified carcinoembryonic antigen in solid-phase radioimmunoassay and by Western Blot analysis. The antibody was tested by fluorescence-activated cell sorting for cell surface binding to the LS174T WiDr and HT-29 colon carcinoma cell lines. The antibody was tested by FACS for binding to the surface of normal human polymorphonuclear leukocytes where it appeared nonreactive (Muraro et al., 1985; PMID: 2413997). The antibody was shown to bind CEA with a Ka of 1.4 x 10^9 M^-1 (Kuroki et al., 1989; PMID: 2474516). The antibody was used for immunohistochemistry on colorectal, gastric, and lung adenocarcinoma tumor tissues. In addition, pancreatic, breast, gallbladder, bile duct, malignant ovarian teratoma, mucinous endometrial carcinoma, colon cancer, remote colon mucosa and bladder cancer stained positive (Shi et al., 1994; PMID: 7520463 and Robbins et al., 1993; PMID: 8386136). |
Antibody First Published | Muraro et al. Definition by monoclonal antibodies of a repertoire of epitopes on carcinoembryonic antigen differentially expressed in human colon carcinomas versus normal adult tissues Cancer Res. 1985 Nov;45(11 Pt 2)：5769-80. PMID：2413997 |
Note on publication | The original paper describes the generation and characterization of a set of antibodies. The antibodies show selective reactivity for human colon carcinomas. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
