Key features and details | |
Cat. No. | MABL-476 |
Name | Anti-B7-H4 mAbs |
Clone No. | AFD- 6H3 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | functional assay, ELISA, FC, IHC |
Species Reactivity | Human |
Basic Information | |
Specificity | This antibody is specific for the IgC region of human B7-H4. |
Alternative Name | B7H4; B7 homolog 4; B7h.5; O8E; Immune costimulatory protein B7-H4; B7S1; Protein B7S1; B7x; T-cell costimulatory molecule B7x; VTCN1; V-set domain-containing T-cell activation inhibitor 1 |
UniProt | Q7Z7D3 |
Immunogen | The original antibody was raised by immunizing a DO11.10 mouse with B7-H4. |
Application Notes | Humanized variants of this antibody 6H3V6 and 6H3V7 are available on request. This antibody is able to neutralize the effect of an B7-H4 blocking antibody (US9574000). Flow cytometry was performed on HEK293 cells that expressed full length human B7-H4 using this antibody (US9574000). This antibody induces robust internalization of B7-H4 expressed on the surface of both the B7-H4 transfected cell line and the B7-H4 positive breast cancer cell line SK-BR-3 (US9574000). Immunohistochemistry was performed on human tissue using this antibody (US9574000). An internalization assay was performed using this antibody on EG7 (B7-H4 negative cells), EG7.IG7 (B7-H4 low expressing cells), and EG7.IVB3 (B7-H4 high expressing cells) (US9574000). |
Antibody First Published | Langermann et al. Anti-human B7-H4 antibodies and their uses. US9574000 |
Note on publication | |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
