Key features and details | |
Cat. No. | MABL-474 |
Name | Anti-B7-H3 mAbs |
Clone No. | AFD- MJ18 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | Block, FC |
Species Reactivity | Mouse |
Basic Information | |
Specificity | This antibody binds to mouse B7-H3 and does not react with other B7-family molecules. |
Alternative Name | CD276; B7H3 |
UniProt | Q8VE98 |
Immunogen | This antibody was generated by immunizing Sprague Dawley rats with B7-H3-Ig, consisting of the extracellular domain (aa1–242 of mouse B7-H3) linked to the Fc portion of mouse IgG2a, emulsified in CFA (Difco). The antibody was selected by its reactivity to mouse B7-H3-transfected cells, but not to parental cells by flow cytometry. |
Application Notes | This antibody has been used to detect murine B7-H3 by flow cytometry. It blocks B7- H3 signalling and can supress priming of Th2-responses and thereby the development of asthmatic responses in an ovalbumin-induced asthma model in BALB/c mice. |
Antibody First Published | Nagashima et al. B7-H3 contributes to the development of pathogenic Th2 cells in a murine model of asthma. J Immunol. 2008 Sep 15;181(6):4062-71 PMID:18768862 |
Note on publication | Describes the generation and initial characterisation of this antibody. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
