Key features and details | |
Cat. No. | MABL-437 |
Name | Anti-Arg-2 mAbs |
Clone No. | AFD- CPR02 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | in vivo, SPR, ELISA, IF |
Species Reactivity | Human |
Basic Information | |
Specificity | The antibody is specific for Arg-2. Arg-2 is required for MUC2 post-transcriptional synthesis and secretion and, it may play a role in the production of mucus by intestinal cells. |
Alternative Name | Human Anterior Gradient Protein 2 Homolog; Anterior gradient protein 2 homolog; AG-2; hAG-2; HPC8; Secreted cement gland protein XAG-2 homolog |
UniProt | O95994 |
Immunogen | The antibody was isolated from the antibody library after three rounds of panning against residues 41-175 of recombinant human Agr2. |
Application Notes | The scFv fragment of the antibody was able to bind the recombinant human Agr2 protein in ELISA analysis. The binding affinity of the scFv fragment to human Agr2 was measured by surface plasmon resonance (Kd= 4.6×10^−7 M). Immunofluorescence analysis of colon biopsy samples from a healthy donor was performed to verify the ability of CPR02 to recognize the human Agr2 antigen. Results showed the antibody revealed significant staining surrounding the crypts of the intestinal tissue. Further, the antibody was used for immunofluorescent staining of freshly frozen human biopsy samples of Ulcerative Colitis, the antibody produced a strong intensity signal in the colonic crypts region. The antibody was able to recognise the Agr2 antigen in vivo, as demonstrated by the results of the autoradiography of the complete intestinal tract of a mouse having colitis, injected with radiolabelled antibody (US20190309060A1). |
Antibody First Published | |
Note on publication | |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
