Key features and details | |
Cat. No. | MABL-428 |
Name | Anti-Apolipoprotein B-100 mAbs |
Clone No. | AFD- MabB23 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | WB, ELISA |
Species Reactivity | Human |
Basic Information | |
Specificity | This antibody binds human apolipoprotein B (APOB). Apolipoprotein B is a major protein constituent of chylomicrons (apo B-48), LDL (apo B-100) and VLDL (apo B-100). Apo B-100 functions as a recognition signal for the cellular binding and internalization of LDL particles by the apoB/E receptor. It is involved in the metabolism of lipids and is the main protein constituent of lipoproteins such as very low- density lipoprotein (VLDL) and low-density lipoprotein (LDL, the “bad cholesterol”). |
Alternative Name | APOB; Apolipoprotein B-100; Apo B-100 |
UniProt | P04114 |
Immunogen | The original antibody was generated by immunizing female BALB/c mice intraperitoneally with 20ug of human apo B-100 and dead Salmonella microbes. |
Application Notes | The binding characterization of this antibody towards human APO-B was done using ELISA and immunoblotting. This antibody was also used in a serum assay to determine the quantity of apo- B in a sample using competitive ELISA. This antibody was shown to react with isolated human Apo-B as well as low density lipids but not with high density lipids and lipoprotein-deficient serum (Yoon et al., 1993). |
Antibody First Published | Yoon et al. Production of monoclonal antibodies to human apolipoprotein B- 100 and development of a monoclonal antibody-based, competitive enzyme-linked immunoassay for apolipoprotein B. Korean Journal of Lipidology, 1993, vol. 3, no. 2, pp. 229-239. PMID |
Note on publication | Describes the generation and characterization of two antibodies against human apolipoprotein B-100. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
