Anti-Amiloride mAbs

Cat. No.

MABL-398

Name

Anti-Amiloride mAbs

Clone No.

AFD- BA7.1

From

Recombinant Antibody

Isotype

Engineer antibody

Application

immunoblot, ELISA

Species Reactivity

Human

Basic Information

Specificity

The antibody is specific for the 3,5-diaminopyrazinyl moiety of amiloride. Analogs with a benzyl on the 5-positition of the pyrazine ring or with the subsitution of an iodo for the chloro group in the 6- position of the ring don't bind. An analog with a benzyl substituent on a terminal nitrogen of the guanidinium group has an affinity for the antibody similar to amiloride.

Alternative Name

UniProt

Immunogen

The antibody was raised by immunising BALB/c   mice with an intraperitoneal injection of an amiloride analogue coupled to   BSA.

Application Notes

The antibody has been characterised using   immunoblot analysis (Lin et al. 1994) and ELISA.

Antibody First Published

Kleyman et al. Distinct epitopes on   amiloride Am. J. Physiol. 257 (Cell    C1135-C1141, 1989 PMID:2481976

Note on publication

Describes the generation of polyclonal and monoclonal antibodies for amiloride

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity purified

Buffer

PBS with 0.02% Proclin 300.

Concentration

1 mg/ml.

Storage Recommendation

Store at 4⁰C for up to 3 months. For   longer storage, aliquot and store at - 20⁰C.