Key features and details | |
Cat. No. | MABL-395 |
Name | Anti-alpha-V integrin mAbs |
Clone No. | AFD- EM01309 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | WB, ELISA, FC, IHC |
Species Reactivity | Human |
Basic Information | |
Specificity | This pan-αv antibody is specific for the extracellular domain (amino acids 31 - 992) of the αv chain in all αv-containing heterodimeric complexes. |
Alternative Name | CD51; Vitronectin receptor subunit alpha; av |
UniProt | P06756 |
Immunogen | This antibody was raised by immunising rabbits, isolating B cells from the immunised rabbits and fusing these with a rabbit plasmacytoma cell line (established by Spieker-Polet et al., 1995) to produce stable hybridomas |
Application Notes | This pan-αv-specific antibody has been used to detect αv integrin chains using ELISA and on Western blots (Goodman et al, 2012), and to specifically stain αv integrins in FFPE/TMA material (Goodman et al, 2012; Silginer et al, 2016; Roth et al, 2013). Murine antibodies are typically unable to detect FFPE-embedded integrins. |
Antibody First Published | Goodman et al. Matched rabbit monoclonal antibodies against αv-series integrins reveal a novel αvβ3-LIBS epitope, and permit routine staining of archival paraffin samples of human tumours Biol Open. 2012 Apr 15; 1(4): 329–340. PMID:23213423 |
Note on publication | Describes the generation and characterisation of this antibody. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
