Key features and details | |
Cat. No. | MABL-385 |
Name | Anti-Alpha-ketoglutarate-dependent dioxygenase FTO mAbs |
Clone No. | AFD- 5-2H10 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | ICC, WB |
Species Reactivity | Rat, Human, Mouse |
Basic Information | |
Specificity | This antibody is specific for the alpha-ketoglutarate-dependent dioxygenase FTO. This protein is a RNA demethylase that mediates oxidative demethylation of different RNA species, such as mRNAs,tRNAs and snRNAs, and acts as a regulator of fat mass, adipogenesis and energy homeostasis. Specifically demethylates N6-methyladenosine (m6A) RNA, the most prevalent internal modification of messenger RNA (mRNA) in higher eukaryotes. |
Alternative Name | FTO; Fat mass and obesity-associated protein; U6 small nuclear RNA (2'-O-methyladenosine-N(6)-)-demethylase FTO; U6 small nuclear RNA N(6)-methyladenosine-demethylase FTO; mRNA (2'-O-methyladenosine-N(6)-)-demethylase FTO; m6A(m)-demethylase FTO; mRNA N(6)-methyladenosine demethylase FTO; tRNA N1-methyl adenine demethylase FTO |
UniProt | Q9C0B1 |
Immunogen | This antigen was raised by immunizing a BALB/c mouse with the alpha-ketoglutaratedependent dioxygenase FTO antigen. |
Application Notes | This antibody was used to assess the expression of the FTO protein in cell line 3T3-L1.The method used was a western blot (Jiao et al, 2016; pmid: 26907332). Immunocytochemistry with this antibody was used for examination of FTO expression in HOC313, MDA-MB-231, SAS, HeLa, 293FT, and MKN45 cells. The experiment was preformed on normal cells and FTO knock-out cells (Hirayama et al,2020; pmid：32268083). |
Antibody First Published | Jiao et al. The Fto Gene Regulates the Proliferation and Differentiation of Pre-Adipocytes in Vitro Nutrients. 2016 Feb 19;8(2): 102. PMID: 26907332 |
Note on publication | The potential role of Fto gene on the proliferation and differentiation of preadipocytes was investigated. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
