Anti-alpha-Gal mAbs

Cat. No.

MABL-383

Name

Anti-alpha-Gal mAbs

Clone No.

AFD- Gal13

From

Recombinant Antibody

Isotype

Engineer antibody

Application

ELISA

Species Reactivity

Cow, Rabbit

Basic Information

Specificity

This antibody is specific for α-gal epitopes (Galα1–3Galβ1–4GlcNAc-R) on glycosphingolipids.

Alternative Name

alpha gal; Galα1–3Galβ1–4GlcNAc-R; alpha gal; gal; α-gal

UniProt

Immunogen

This antibody was raised by immunising BALB/C mice, which express α-gal epitopes, with rabbit red cell membranes, on  which α-gal is the major carbohydrate epitope.

Application Notes

This antibody binds to synthetic α-gal   epitopes linked to BSA and to α-gal epitopes on glycolipid molecules from   rabbit and bovine erythrocytes (Chen et al, 2000; Galili et al, 1987).   However, in contrast to monoclonal anti-Gal antibodies originating from  α1,3GT knockout mice [M86; B1H2; B4H2], this antibody does not bind to α-gal epitopes on carbohydrate chains of natural glycoproteins. This antibody has   been used to confirm that Galαl-3Gal glycosphingolipids are expressed at low levels by human erythrocytes (Galili et al, 1987).

Antibody First Published

Galili et al. Identification of erythrocyte Gal alpha 1-3Gal glycosphingolipids with a mouse monoclonal antibody, Gal-13.   J Biol Chem. 1987 Apr 5;262(10)：4683-8. PMID: 2435715

Note on publication

Describes the original generation of this antibody, and its characterisation in ELISA analysis.

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity purified

Buffer

PBS with 0.02% Proclin 300.

Concentration

1 mg/ml.

Storage Recommendation

Store at 4⁰C for up to 3 months. For   longer storage, aliquot and store at - 20⁰C.