Key features and details | |
Cat. No. | MABL-378 |
Name | Anti-Alpha-Fetoprotein mAbs |
Clone No. | AFD- 9H3 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | functional assay, ELISA |
Species Reactivity | Human |
Basic Information | |
Specificity | This antibody binds human alpha-fetoprotein (AFP). AFP is made in the liver of developing babies. It binds copper, nickel, and fatty acids as well as, and bilirubin less well than, serum albumin. AFP levels in healthy adults is very low. Elevated levels of AFP in adult human blood is a marker for cancer. |
Alternative Name | AFP; Alpha-fetoglobulin; A-Feto-protein |
UniProt | P02771 |
Immunogen | The original antibody was generated by immunizing BALB/c mice with human alphafetoprotein.The variable region genes of the antibody were used to generate the scFv version of this antibody. |
Application Notes | The binding characterization of this antibody to human alpha-fetoprotein was done using ELISA. The inhibitory effect of single-chain antibody against alpha-fetoprotein-positive liver cancer cells was studies and it was found that the scFv antibody has a significant growth inhibitory effect on AFPpositive HepG2 and Bel7402. The scFv antibody was also found to significantly increase the sensitivity of AFP-positive HepG2 cells to all-trans retinoic acid (CN103319595). |
Antibody First Published | |
Note on publication | |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
