Key features and details | |
Cat. No. | MABL-375 |
Name | Anti-Alpha-crystallin mAbs |
Clone No. | AFD- B4m3 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | SPR, ELISA |
Species Reactivity | Mycobacterium tuberculosis |
Basic Information | |
Specificity | This antibody is specific for the HspX antigen from Mycobacterium tuberculosis. This antigen acts as a chaperone, as it has a significant ability to suppress the thermal denaturation of alcohol dehydrogenase. Mycobacterium tuberculosis causes the disease tuberculosis. |
Alternative Name | Acr; hspX; 14 kDa antigen; 16 kDa antigen; Antigen 16kDa (HspX); HSP 16.3; Nox16 |
UniProt | P9WMK1 |
Immunogen | This antibody was generated by first panning the Nkuku® library [26] against 20 μg/ml of the M. tuberculosis 16 kDa antigen. Second a sublibrary was created using error-prone PCR. Finally the mutant library was panned against 16 kDa M. tuberculosis antigen. |
Application Notes | The binding of the scFv version of this antibody to the antigen was characterised by ELISA. Furthermore, surface plasmon resonance was used to determine the binding kinetics of this antibody on 16 kDa protein of M. tuberculosis (Sixholo et al, 2011; pmid:21349739). |
Antibody First Published | Sixholo et al. Improving the characteristics of a mycobacterial 16 kDaspecific chicken scFv. Biologicals. 2011 Mar;39(2):110-6. PMID:21349739 |
Note on publication | A chicken single chain variable fragment (scFv) phage display library was panned against the mycobacterial 16 kDa antigen. Three fusion phages which bound specifically to the antigen were selected, each of which produced low signals in ELISA when secreted as a soluble scFv. One scFv was therefore chosen to be modified in an attempt to improve its binding. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
