Key features and details | |
Cat. No. | MABL-367 |
Name | Anti-Alpha Actinin 4 mAbs |
Clone No. | AFD- Ab01AA4 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | IP, ELISA, IHC |
Species Reactivity | Human |
Basic Information | |
Specificity | This antibody specific for an ALPHA-ACTININ-4 antigen, including the heavy chain CDR antigen binding site amino acid sequences CDR1, CDR2, and CDR3. |
Alternative Name | Non-muscle alpha-actinin 4 |
UniProt | O43707 |
Immunogen | The original antibody was raised by immunizing mice with human breast cancer cell line SKBR3. |
Application Notes | A protein obtained using this antibody is digested with trypsin and analyzed by MALDIMS. From the analysis it was determined that this protein was alpha-actinin-4 (US9040018). An 8% Trisglycine gel shows recombinant alpha-actinin-4 protein, and soluble alpha-actinin-4 protein from KATO-3 conditioned cell medium isolated with this antibody (US9040018). ELISA was performed using this antibody on plasma samples (Control: plasma from healthy people, Breast Cancer: plasma from breast cancer patients) from control and patient groups (US9040018). Immunohistochemistry is performed with this antibody using a Actinin-4 IHC Kit on a total of 714 breast tumor and control samples (US9040018). |
Antibody First Published | Alper Ozge Monoclonal antibodies against alpha-actinin-4 antigens, and uses therefor. US9040018 PMID |
Note on publication | |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
