Key features and details | |
Cat. No. | MABL-362 |
Name | Anti-AIF1 mAbs |
Clone No. | AFD- GT1-mAb1 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | WB, ELISA, FC, IF |
Species Reactivity | Human |
Basic Information | |
Specificity | This antibody is specific to EBP05419, an immunizing peptide with the sequence CTGPPAKKAISELP. This peptide was used in the production of the polyclonal Goat Anti-AIF1 antibody from which this antibody is derived. |
Alternative Name | AIF1; IBA1; allograft inflammatory factor 1; AIF-1; IRT-1;interferon gamma responsive transcript; ionized calcium-binding adapter molecule 1; Daintain; DADB-70P7.8; allograft inflammatory factor-1 splice variant Hara-1; IRT1; protein G1 |
UniProt | P55008 |
Immunogen | This antibody was obtained by sequencing a polyclonal goat IgG raised against a peptide with the sequence C-TGPPAKKAISELP. |
Application Notes | This antibody (GT1-mAb1) was one of two monoclonal antibodies discovered by analyzing a polyclonal antibody (GT-1) sample. Briefly, de novo sequencing of GT-1 resulted in the fulllength sequences of two distinct monoclonal antibodies (GT1-mAb1 and GT1-mAb2) in both framework and CDR regions. Both antibodies were recombinantly expressed by Absolute Antibody in HEK293 cells and tested in various assays. GT1-mAb1 was tested against the peptide antigen in an ELISA, and it showed activity comparable to that of the original polyclonal antibody. GT1-mAb1 was found to bind the target peptide in a non-competitive manner with GT1-mAb2 while being efficiently blocked by the original polyclonal antibody, proving that both monoclonal antibodies bind distinct epitopes and that GT1-mAb1 possess binding characteristics of the input polyclonal antibody. Furthermore, GT1-mAb1 was compatible with immunofluorescence (IF), flow cytometry (FC), and Western blot (WB), with an FC signal observedly more distinct than that of GT1-mAb2. |
Antibody First Published | |
Note on publication | |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
