Anti-ABCB1 mAbs

Cat. No.

MABL-336

Name

Anti-ABCB1 mAbs

Clone No.

AFD-C219

From

Recombinant Antibody

Isotype

Engineer antibody

Application

crystallization, WB, ELISA, IHC

Species Reactivity

Chinese Hamster, Dog, Human

Basic Information

Specificity

This antibody is specific for the   epitope VQ(E/A)ALD of Chinese hamster ABCB1.

Alternative Name

CD243; P-glycoprotein 1; PGP1; PGY1; Multidrug resistance protein 1; Phospholipid transporter ABCB1; ATP-dependent translocase ABCB1; ATP-binding cassette sub-family B member 1

UniProt

P21448

Immunogen

The original antibody was raised against   plasma membranes of multidrug-resistant Chinese hamster ovary cells.

Application Notes

The original antibody was used for a western blot on whole cell extracts of E. coli cells overexpressing a fusion   of the carboxyl-terminal NBD of Pgp to maltose-binding protein. The structure of the original antibody was determined using x-ray crystallography (Hoedemaeker et al., 1997; PMID:9368049, van Den Elsen et al., 1999; PMID:10570132). This antibody was used for immunohistochemistry on breast cancer cell lines, to determine the cross reactivity of this antibody with   p185. This antibody was used for an ELISA on different peptides from p170 and p185 (Liu et al., 1997; PMID:9337349). This antibody was used for immnohistochemistry on high-grade lymphoma from dogs (Bergman et al., 1996;   PMID:8947866), and on Ovarian Epithelium, Fallopian Tube, and Ovarian   Carcinoma Specimens (Finstad et al., 1991; PMID:1682363).

Antibody First Published

Finstad et al. Some monoclonal antibody reagents (C219 and JSB-1) to Pglycoprotein contain antibodies to   blood group A carbohydrate determinants: a problem of quality control for   immunohistochemical analysis J Histochem Cytochem. 1991 Dec;39(12):1603-10.   PMID:1682363

Note on publication

The original study demonstrates that   some commercial lots of these MAb, even those supplied as purified   immunoglobulins, contain contaminating anti-A blood group antibodies.

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity purified

Buffer

PBS with 0.02% Proclin 300.

Concentration

1 mg/ml.

Storage Recommendation

Store at 4⁰C for up to 3 months. For   longer storage, aliquot and store at - 20⁰C.