Key features and details | |
Cat. No. | MABL-314 |
Name | Anti-4-1BB mAbs |
Clone No. | AFD-4B4-1-1 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | Block, ELISA, FC, IHC |
Species Reactivity | Human |
Basic Information | |
Specificity | This antibody binds to the human 4-1BB, which is expressed on the surface of activated T cells. |
Alternative Name | CD137; MGC2172; Tumor necrosis factor receptor superfamily member 9 (TNFRSF9); T cell antigen ILA; Interleukin activated receptor; 4-1BB ligand receptor CDw137; T-cell antigen 4-1BB homolog; T-cell antigen ILA. |
UniProt | Q07011 |
Immunogen | The original antibody was generated by immunizing BALB/c mice with a fusion protein of human 4-1BB antigen and glutathione s transferase (GST). |
Application Notes | The antibody has good binding affinity for Con A-activated T cells and has an immunosuppressive activity towards lymphocyte proliferation in the in vitro mixed lymphocyte reaction. The binding of antibody to surface expressed 4-1BB molecule was assessed using flow cytometry and ELISA was used to check whether the cell culture had binding affinity to 4-1BB (Kim et al., 1995). The interaction of this antibody with CD137 is reported to induce an increase in TNF-α production by monocytes. This antibody competes with mAb M121 for binding to CD137 (PMID: 10607752). This antiboy also induces the production of IFN-γ in NK cells in presence of IL-15 (PMID: 20008791).This antibody can be used in blocking assays and staining methods (PMID: 14746806). |
Antibody First Published | Kim et al. Immunoregulatory effects of a monoclonal antibody to human 4-1 bb molecule on alloantigen-mediated immune responses. Proceedings of the Korean Society of Applied Pharmacology (1995), pg 82-82. PMID: |
Note on publication | Describes the generation of the antibody. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
