Anti-4-1BB mAbs

Cat. No.

MABL-313

Name

Anti-4-1BB mAbs

Clone No.

AFD- 3H3

From

Recombinant Antibody

Isotype

Engineer antibody

Application

IP, stimulatory, ELISA

Species Reactivity

Mouse

Basic Information

Specificity

This antibody is specific for murine 4-1BB, an inducible type I membrane protein that is rapidly expressed on the surface of CD4+ and CD8+ T cells after activation.

Alternative Name

CD137; 4 1BB; 41BB; Tumor necrosis factor receptor superfamily member 9; Tnfrsf9; T-cell antigen 4-1BB; Ila, Ly63; 4-1BB receptor

UniProt

P20334

Immunogen

This antibody was raised by   immunizing Lewis rats with 4-1BB fusion proteins, composed of the extracellular domain of murine 4-1BB and the COOH region of human IgG1 .

Application Notes

The binding specificity of this   antibody for 4-1BB has been confirmed in ELISA analysis using purified 4-1BB–Ig fusion protein, and immunoprecipitation analysis (Shuford et al,   1997).This antibody is able to block 4-1BBL binding to activated DO.11.10 T cells   (Shuford et al, 1997). This antibody reacts with activated T cells, and can   costimulate T cell proliferation induced by the 145.2C11 hamster anti– mouse   CD3 mAb (Shuford et al, 1997). Equally, when administered to mice, this   antibody expands innate immune cells, as well as host T cells (Lin et al, 2010). Administration of this antibody alone induced complete tumor   regression in E.G7 tumor-bearing mice (Lin et al, 2010). Binding of this   antibody to activated T cells in mice receiving heart allografts led to the   rapid rejection of the allograft (Shuford et al, 1997).

Antibody First Published

Shuford et al. 4-1BB costimulatory   signals preferentially induce CD8+ T cell proliferation and lead to the   amplification in vivo of cytotoxic T cell responses. J Exp Med. 1997 Jul   7;186(1):47-55. PMID:9206996

Note on publication

Describes the original generation of   this antibody, and its characterisation in ELISA analysis.

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity purified

Buffer

PBS with 0.02% Proclin 300.

Concentration

1 mg/ml.

Storage Recommendation

Store at 4⁰C for up to 3 months. For   longer storage, aliquot and store at - 20⁰C.