Key features and details | |
Cat. No. | MABL-282 |
Name | Anti-1-pHis mAbs |
Clone No. | AFD-SC1-1 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | functional assays, Immunoaffinity purification, IP, WB, IF |
Species Reactivity | Species independent |
Basic Information | |
Specificity | This antibody is specific for 1-pHis and does not cross react with phosphotyrosine (pTyr) or the other pHis isomers. |
Alternative Name | 1-phosphohistidine; N1-Phosphohistidine; histidine |
UniProt | |
Immunogen | The original rabbit version of this antibody was raised by immunizing rabbits with a 1-pTza library conjugated to the carrier protein keyhole limpet hemocyanin (KLH). |
Application Notes | This antibody can be used for the identification of 1-pHis substrates and functional study of pHis. It was used to assess pHis levels in lysates of various cell lines, such as HPDE6 and pancreatic cancer, FLAG-NME1 293, HeLa, C2C12, and NME1- and NME2-overexpressing (OE) melanoma cell lines via Western blot (Fuhs et al, 2015; pmid: 26140597). A dot blot was also preformed on rabbit antisera, the 1-pTza, 3-pTza peptide libraries, His control library and a pTyr peptide with this antibody demonstrating the antibody's specificity to 1-pHis (Fuhs et al, 2015; pmid:26140597). Phosphorylation of H118 on NDPK-B was assessed as well by immunoblotting with this antibody (Panda et al, 2016; pmid:27453048). SC1-1 was further used to identify the intracellular location of pHis in HeLa cells via immunofluorescence. This clone was shown to be useful for studies on the acidic acidic compartments (i.e., phagosomes, autophagosomes, and lysosomes) since staining is absent from the interior of these acidic compartments and often very bright in the surrounding regions (Fuhs et al, 2015; pmid:26140597). |
Antibody First Published | Fuhs et al. Monoclonal 1- and 3-Phosphohistidine Antibodies: New Tools to Study Histidine Phosphorylation Cell. 2015 Jul 2; 162(1): 198–210. PMID:26140597 |
Note on publication | Describes the generation and characterization of the monoclonal antibodies (mAbs) that specifically recognize 1-pHis or 3-pHis. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
