Anti-1-4 beta-d-galactan mAbs

Cat. No.

MABL-281

Name

Anti-1-4 beta-d-galactan mAbs

Clone No.

AFD-XD3

From

Recombinant Antibody

Isotype

Engineer antibody

Application

ELISA

Species Reactivity

Zinnia elegans

Basic Information

Specificity

This antibody recognises (1→4)-β-d-galactan. The epitope recognised is present on the primary wall   of tracheary-element precursor cells, but absent from mature tracheary   elements.

Alternative Name

14bdGal; (1→4)-β-d-galactan

UniProt

Immunogen

This antibody was isolated from a   phage-display library raised against a cell wall fraction of Zinnia elegans   xylogenic culture cells. Cell wall fractions were isolated from Zinnia   mesophyll cells that had been cultured either in a differentiation-inducti

Application Notes

Whole-cell ELISA against cultured   plant cells has confirmed that this antibody is differentiation-specific, as the epitope recognised by this antibody   increased specifically on cells cultured in a differentiation-inductive   medium but not on those cultured in a noninductive medium (Shinohara et al,   2002).Equally, this antibody has been shown in ELISA analysis to recognise   (1→4)-β-d-galactan in a pectic fraction (Shinohara et al, 2015).

Antibody First Published

Shinohara et al. Isolation of   monoclonal antibodies recognizing rare and dominant epitopes in plant vascular cell walls by phage display subtraction J Immunol Methods. 2002   Jun 1;264(1-2):187-94. PMID:12191521

Note on publication

Describes the original generation of   this antibody, and its characterisation in ELISA analysis.

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity purified

Buffer

PBS with 0.02% Proclin 300.

Concentration

1 mg/ml.

Storage Recommendation

Store at 4⁰C for up to 3 months. For   longer storage, aliquot and store at - 20⁰C.