Key features and details | |
Cat. No. | MABL-3058 |
Name | Anti-Thomsen-nouveaupolysaccharide A1 mAbs |
Clone No. | AFD- KT-IgM-8 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | Complement‑dependent cytotoxicity, glycan array technologies, in vitro, in vivo, ELISA, FC |
Species Reactivity | Human |
Basic Information | |
Specificity | The antibody is specific for Tn-PS A1. It does not cross react with PS A1. |
Alternative Name | Tn-PS A1; Thomsen-nouveau (Tn); Tn antigen; Tn saccharide; N-acetyl Galactosamine; Alpha-GalNAc; Alpha GalpNAc; GalNAc; GalNAc-alpha; N-acetyl-alpha-D-galactosamine; Blood group A antigen |
UniProt | |
Immunogen | The original antibody was generated by immunizing C57BL/6 mice against Tn-PS A1. |
Application Notes | The specificity of the original format of the antibody was confirmed by ELISA analysis. The ability of the antibody to bind cancer cells expressing the Tn antigen on the cell surface (MCF-7 and HCT-116) was evaluated by by FC. The antibody demonstrated the ability to bind both MCF-7 and HCT-116 tumor cells at 30 µg/mL with a shift in fluorescence of 49% in both cell lines. The antibody demonstrated in vitro tumor killing using an LDH assay. The assay measured that the antibody-induced complement- dependent cytotoxicity was 30%. In vivo passive immunotherapy approach using an MCF-7 cell line-derived xenograft model was evaluated. The antibody demonstrated approximately 40% reduction in tumor growth in the MCF-7 model (Trabbic et al., 2018; PMID: 30030557). |
Antibody First Published | Trabbic et al. Production of a mouse monoclonal IgM antibody that targets the carbohydrate Thomsen-nouveau cancer antigen resulting in in vivo and in vitro tumor killing Cancer Immunol Immunother. 2018 Sep;67(9):1437-1447. PMID:30030557 |
Note on publication | The original paper describes the generation and characterization of the antibody. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
