Key features and details | |
Cat. No. | MABL-2941 |
Name | Anti-SN-38 mAbs |
Clone No. | AFD- 1H3-1 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | SPR, ELISA |
Species Reactivity | Species independent |
Basic Information | |
Specificity | This antibody binds SN-38, which is an active metabolite of irinotecan (a semisynthetic analog of camptothecin - a topoisomerase I inhibitor) generated by carboxyl esterases through hydrolysis. SN-38 has potential antineoplastic activity. It binds and inhibits topoisomerase I by stabilizing the cleavable complex between topoisomerase I and DNA, resulting in DNA breaks, inhibition of DNA replication, and apoptosis. In vitro cytotoxicity assays have shown that SN-38 is 1,000-times more cytotoxic than irinotecan against various cancerous cells. SN-38 is commonly used as a cytotoxic drug payload in the ADC field. |
Alternative Name | SN38; Topotecan; Camptothecin; CPT; 9-[(dimethylamino)methyl]-10- hydroxy-(4S)-camptothecin; 10-Hydroxy-7-ethylcamptothecin; 7-Ethyl-10-hydroxy-20(S)-camptothecin; 7- Ethyl-10-hydroxycamptothecin; NK 012; NK-012 |
UniProt | |
Immunogen | The original antibody was generated by immunizing BALB/c mice with SN38-BSA conjugate. |
Application Notes | The binding reactivity of this antibody to SN38-BSA conjugate was determined using ELISA. The binding affinity of this antibody to SN38-KLH conjugate was determined using surface plasmon resonance (SPR). It was reported that this antibody binds SN38-KLH conjugate with a binding affinity of Kd=3.06 nM (CN115197323). |
Antibody First Published | |
Note on publication | |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
