Key features and details | |
Cat. No. | MABL-2906 |
Name | Anti-Sialoadhesin mAbs |
Clone No. | AFD- 3B11 (3B11/11) |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | IP, WB, FC, IF, IHC |
Species Reactivity | Pig (Sus scrofa) |
Basic Information | |
Specificity | This antibody recognizes and binds porcine sialoadhesin. |
Alternative Name | CD169; pSn; Sn; p210; Siglec-1; Sialic acid-binding Ig-like lectin 1 |
UniProt | A7LCJ3 |
Immunogen | The original antibody was generated by immunizing BALB/c mice with porcine alveolar macrophages. |
Application Notes | This antibody is recommended to be used as a tool in the study of porcine macrophage maturation and differentiation and for determining their heterogeneity both in normal and pathological conditions. This antibody can recognize porcine sialoadhesin in a western blot assay and an immunoprecipitation assay (PMID: 9151393). Tissue distribution of the antigen recognized by this mAb was analyzed by immunohistochemical staining of different tissues (PMID:19081005). 3B11 successfully binds alveolar macrophages and PAM cells in a flow cytometric analysis (PMID:19081005, 23785195). Porcine sialoadhesin has also been analyzed as potential antigen targeting receptors using mouse IgG as surrogate antigens (PMID:21557980). The mAb was also used in the study the role of sialoadhesin in porcine reproductive and respiratory syndrome virus infection using immunoflurosence and flow cytometric analysis (Liu et al, 2020) |
Antibody First Published | Bullido et al. Monoclonal antibodies specific for porcine monocytes/macrophages: macrophage heterogeneity in the pig evidenced by the expression of surface antigens. Tissue Antigens (1997); 49(4):403-13. PMID:9151393 |
Note on publication | Describes the distribution of surface antigens on porcine monocytes and macrophages using monoclonal antibodies. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
