Anti-ribosomal P protein mAbs

Cat. No.

MABL-2833

Name

Anti-ribosomal P protein mAbs

Clone No.

AFD- B10

From

Recombinant Antibody

Isotype

Engineer antibody

Application

functional assays

Species Reactivity

Trypanosoma cruzi

Basic Information

Specificity

This antibody recognises DDxGF sequence of the C-terminal of eukaryotic ribosomal P proteins.

Alternative Name

60S acidic ribosomal protein P2-B; P2B; R13;   P2b; TcP2b; R13

UniProt

P26795

Immunogen

This antibody was derived from BALB/c mice   immunised with TcP2b.

Application Notes

Both B10 mAb and scFv were demonstrated to detect eukaryote ribosomal P proteins with a high specificity demonstrated by the alanine-scanning mutagenesis of peptide R13 (López Bergami et al., 2003). Consequently, this antibody can be used in the detection of ribosomal P   proteins in various eukarote tissues. Finally, anti-P protein antibodies are   observed in certain pathologies, such as Trypanosoma cruzi infection and SLE, and thus B10 antibody might be utilised as a model for the investigation of   the immunology of these diseases (López Bergami et al., 2003).

Antibody First   Published

López Bergami et al. Sequence analysis,   expression, and paratope characterization of a single-chain Fv fragment for   the eukaryote ribosomal P proteins. Biochem Biophys Res Commun. 2003 Feb   21;301(4):819-24. PMID:12589786

Note on publication

This article describes the generation and   characterisation of the B10 monoclonal antibody as well as its scFV   derivative.

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified   antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity   purified

Buffer

PBS with 0.02% Proclin   300.

Concentration

1 mg/ml.

Storage   Recommendation

Store at 4⁰C for up to 3   months. For longer storage, aliquot and store at - 20⁰C.