Key features and details | |
Cat. No. | MABL-2820 |
Name | Anti-Reticulon-1A/B mAbs |
Clone No. | AFD- RNL-3 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | WB, FC, IHC |
Species Reactivity | Monkey, Rabbit, Human |
Basic Information | |
Specificity | The antibody binds Reticulon-1A/B / NSP-A/B. The antibody recognizes an epitope located within the region of amino acids 421-589 of the neuro-endocrine specific protein Reticulon-1A (NSP-A), which is also present in the N-terminal part of Reticulon-1B (NSP-B). |
Alternative Name | NSP-A/B; Reticulon-1; Neuroendocrine-specific protein |
UniProt | Q16799 |
Immunogen | The original antibody was generated by immunizing a BALB/c mouse with the small cell lung cancer cell line NCI-H82. |
Application Notes | The antibody detected NSP-A and B from NCI-H82 cells by western blot analysis. Immunofluorescence using the antibody revealed NSPs localized in the endoplasmic reticulum (ER) of NCI- H524 cells and migrated to the ER of COS-1 cells after transfection with the NSP-B construct. The antibody recognized a sub-set of neuro-endocrine tissues and tumours by immunohystochemistry. In normal tissues, the antibody reacted with brain Purkinje cells, pancreatic islet cells, some cells in the pituitary gland and some (peripheral) nerve fibres. Further, spleen reticular fibres, prostatic epithelium and some suprabasal cells of the tongue epithelium were also positive with the antibody. |
Antibody First Published | Broers et al. Novel antigens characteristic of neuroendocrine malignancies Cancer. 1991 Feb 1;67(3):619-33. PMID:1845952 |
Note on publication | The original paper describes the generation and characterization of the antibody. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
