Anti-RAGE mAbs

Cat. No.

MABL-2796

Name

Anti-RAGE mAbs

Clone No.

AFD- 2A11

From

Recombinant Antibody

Isotype

Engineer antibody

Application

SPR, Western blot, ELISA, FC, IF, IHC

Species Reactivity

Human

Basic Information

Specificity

2A11 is specific for the V-domain of human   advanced glycosylation end product-specific receptor. It recognises both the   glycosylated and non-glycosylated forms.

Alternative Name

AGER; advanced glycosylation end   product-specific receptor; receptor for advanced glycosylation end products

UniProt

Q15109

Immunogen

Human RAGE protein.

Application Notes

ELISA confirmed the specificity of 2A11 for the   V-domain of RAGE, with a binding affinity (Kd) of 7.6 nM. ELISA was also used   to confirm that the binding of 2A11 to RAGE is glycosylation independent. In   SPR experiments the Kd of 2A11 binding to RAGE was determined as 0.38 nM.   Flow cytometry and immunochemistry were used to visualise the binding of 2A11   to HEK-RAGE cells.

Antibody First   Published

Jyoti, 2012. Development of new antibody   based theranostic agents targeting the receptor for advanced glycation   end-product (RAGE). Dissertation Submitted to the Graduate Faculty of the   North Dakota State University of Agriculture and Applied Science

Note on publication

Describes the characterisation of 2A11   through ELISA, SPR, FC, and IH experiments.

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified   antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity   purified

Buffer

PBS with 0.02% Proclin   300.

Concentration

1 mg/ml.

Storage   Recommendation

Store at 4⁰C for up to 3   months. For longer storage, aliquot and store at - 20⁰C.