Key features and details | |
Cat. No. | MABL-2792 |
Name | Anti-Rad51C mAbs |
Clone No. | AFD- 2H11/6 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | IP, WB, IF, IHC |
Species Reactivity | Primate, Yeast, Human, Mouse |
Basic Information | |
Specificity | This antibody recognises Rad51C in human, mouse, primate and yeast. Rad51C is a Rad51 paralog, which is a homologue of RecA (E. coli). It is involved in homologous recombination during DNA replication and repair. |
Alternative Name | DNA repair protein RAD51 homolog 3; R51H3; RAD51 homolog C; RAD51- like protein 2; BROVCA3; FANCO; MGC104277; RAD51-like protein 2; yeast RAD51 homolog 3; RAD51L2RAD51 (S. cerevisiae) homolog C; RAD51L2; 2H11 |
UniProt | O43502 |
Immunogen | This antibody was raised by immunising mice with the denaturated recombinant human RAD51C-His10 protein which had been purified from 2.4 liters of E. coli FB810 recA− pLysS carrying pRAD51C-16b. |
Application Notes | WB: Rad51C 2H11/6 antibody was used to visualise Rad51C on Western blots in multiple experiments (Masson et al., 2001). IF/ICC: This antibody was also used to stain Rad51C in HEK293 cells during experiments aimed at determining the cellular localization of Rad51C (Bennett and Knight, 2005). |
Antibody First Published | Masson et al. Complex formation by the human RAD51C and XRCC3 recombination repair proteins. Proc Natl Acad Sci U S A. 2001 Jul 17;98(15):8440-6. PMID:11459987 |
Note on publication | This article describes the detailed generation of this anti-Rad51C antibody as well as successfully applies it in Western analysis. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
