Anti-PV11 mAbs

Cat. No.

MABL-2776

Name

Anti-PV11 mAbs

Clone No.

AFD- III20

From

Recombinant Antibody

Isotype

Engineer antibody

Application

in vitro neutralization, WB, ELISA, IF

Species Reactivity

Rabies Virus

Basic Information

Specificity

The antibody binds the PV11 strain of the   rabies virus.

Alternative Name

UniProt

Immunogen

The original scFv antibody was selected from a human synthetic scFv phage display library by panning against the PV11  strain of the virus.

Application Notes

The specificity of the scFv-Fc format of the   antibody was confirmed by ELISA analysis. The scFv-Fc version of the antibody   detected GPRV by western blot analysis. The scFv-Fc version of the antibody   bound specifically to the membranes of PV11 infected Vero cells in an   immunofluorescence assay. The scFv-Fc version of the antibody neutralized the   PV11 strain in a standard in vivo neutralization assay where the virus was   incubated with the scFv-Fc fragment before intracranial inoculation in mice   (Ray et al., 2001; PMID: 11472431).

Antibody First   Published

Ray et al. Selection of single chain   variable fragments (scFv) against the glycoprotein antigen of the rabies   virus from a human synthetic scFv phage display library and their fusion with   the Fc region of human IgG1 Clin Exp Immunol. 2001 Jul; 125(1): 94–101.   PMID:11472431

Note on publication

The original paper describes the generation   and characterization of the antibody.

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified   antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity   purified

Buffer

PBS with 0.02% Proclin   300.

Concentration

1 mg/ml.

Storage   Recommendation

Store at 4⁰C for up to 3   months. For longer storage, aliquot and store at - 20⁰C.