Key features and details | |
Cat. No. | MABL-2718 |
Name | Anti-PorA protein mAbs |
Clone No. | AFD- MN14C11.6 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | functional assay, WB, ELISA |
Species Reactivity | Neisseria meningitidis serogroup A and B |
Basic Information | |
Specificity | This antibody recognizes the P1.7 loop of the PorA protein of Neisseria meningitidis. Epitope mapping revealed that this antibody specifically binds a sequence 'ASGQ'. This antibody can bind both A and B serotype of Neisseria meningitidis. The major outer membrane protein PorA of Neisseria meningitidis is the target for bactericidal serosubtyping antibodies and is currently considered as a potential vaccine candidate against group B meningococcal disease. |
Alternative Name | PIA; Protein IA; Class 1 protein; P1.7; P1.7 serosubtype; Major outer membrane protein P.IA; serogroup A; serogroup B |
UniProt | P57041; E6MXW0 |
Immunogen | The original antibody was generated immunizing mice with outer membrane protein preparations from Neisseria meningitidis. |
Application Notes | This antibody showed both bactericidal and opsonophagocytic for P1.7-expressing meningococci. The lowest concentration resulting in >50% killing of strain 44/76-SL was reported to be 0.5 µg/l. The opsonic activity of this antibody was measured against strain 44/76-SL with human peripheral blood polymorphonuclear leukocytes (PMN) as the effector cells and human serum as the complement source. This antibody demonstrated high opsonophagocytic activity with >80% of PMNs exhibiting measurable RB at a concentration of the MAbs of 10 μg/ml. This antibody was opsonic for the test strain 44/76-SL at a concentration of 0.2 μg/ml (PMID: 10722576). This antibody is not capable of binding meningococcal isolates expressing some variations of the P1.7 family especially P1.7b in a whole cell ELISA (PMID: 10512717). This antibody can be used in the detection of outer membrane protein of N. meningitidis strains like 44/76 using an ELISA (PMID: 12753502). |
Antibody First Published | Abdillahi et al. Definition of meningococcal class 1 OMP subtyping antigens by monoclonal antibodies. FEMS Microbiol Immunol. 1988 Dec;1(3):139-44. PMID:3152545 |
Note on publication | The paper defines antigenic determinants on the class 1 outer membrane proteins |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
