Key features and details | |
Cat. No. | MABL-2699 |
Name | Anti-PLA2, Fraction 19 mAbs |
Clone No. | AFD- TPL004_01_A11 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | ELISA |
Species Reactivity | Naja nigricollis |
Basic Information | |
Specificity | TPL004_01_A11 antibody recognizes PLA2 (Phospholipase A2 'basic'), which is one of the major proteins in the venom of the black-necked spitting cobra (Naja nigricollis). |
Alternative Name | Phospholipase A2 'basic'; Phospholipase A2; svPLA2; Phosphatidylcholine 2-acylhydrolase; Phospholipase A2 isozyme III/IV; CM-III/CM-IV |
UniProt | P00605 |
Immunogen | The antibody was raised using IONTAS phage display library, a naïve human scFv library consisting of 4.0 · 10^10 unique antibody fragments. Through phage display selection, scFv-presenting phages were allowed to bind the venom proteins, phages with unspecific binding were washed away, and the rest were eluted through enzymatic digestion. These were then amplified and used for the following round. In total three rounds of selections were completed leaving the best binders behind. |
Application Notes | TPL004_01_A11 antibody has a very high affinity to the PLA2. It was demonstrated by normalized ELISA, which circumvents the limitation of a simple ELISA by removing the expression variable, normalizing the amount of scFv in each well. The antibody's high affinity was confirmed by its ability to bind the target protein in a venom fraction even at 1:32,000 dilution (3.125 · 10^-2) µg/mL (Jürgensen et al., 2019 [poster]). It is recommended for the research on the antitoxin against Naja nigricollis snakebites. |
Antibody First Published | Jürgensen et al. Harnessing monoclonal antibodies for development of a specific treatment against Naja nigricollis envenoming. 10.13140/RG.2.2.24771.14884. |
Note on publication | This poster describes the generation and characterization of the TPL004_01_A11 antibody among other antibodies directed against the venom toxins of Naja nigricollis. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
