Key features and details | |
Cat. No. | MABL-2682 |
Name | Anti-Pharynx mAbs |
Clone No. | AFD- KT22 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | IF |
Species Reactivity | Caenorhabditis elegans |
Basic Information | |
Specificity | This antibody recognized the apical side of the pharynx of Caenorhabditis elegans. Caenorhabditis elegans is a transparent nematode that lives in temperate soil environments. |
Alternative Name | |
UniProt | |
Immunogen | The original antibody was generated by immunizing BALB/c mice with subtracted embryonic extract. Subtracted extracts were prepared by using a method called “antigen subtraction” which enriched antigens of low abundance in heterogeneous molecule mixtures by subtracting abundant antigens. |
Application Notes | This antibody was used for the staining of the apical region of the pharynx of Caenorhabditis elegans. The KT22 signal was detectable in the twofold embryo as a single line from the buccal cavity to the end of isthmus; at the pretzel stage, the KT22 stained three lines from the buccal cavity to the anterior isthmus, which appeared to merge into a single line in the posterior isthmus region. No KT22 signal was detected in the terminal bulb and pharyngeal–intestinal valve (PMID; 18498356). |
Antibody First Published | Takeda et al. Efficient production of monoclonal antibodies recognizing specific structures in Caenorhabditis elegans embryos using an antigen subtraction method. Genes Cells. 2008 Jul;13(7):653-65. PMID:18498356 |
Note on publication | Describes the use of "antigen subtraction" method to enrich antigens of low abundance in heterogeneous molecule mixtures by subtracting abundant antigens. The paper also discloses generation of 35 mAbs against Caenorhabditis elegans that recognize specific structures, including P granules, muscles, the pharynx, and subsets of hypodermal cells; some of the mAbs revealed previously unreported cellular structures. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
