Key features and details | |
Cat. No. | MABL-2627 |
Name | Anti-PCSK9 mAbs |
Clone No. | AFD- PA4 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | inhibit, therapeutic, WB, Block, ELISA |
Species Reactivity | Human |
Basic Information | |
Specificity | This antibody binds human PCSK9. Proprotein convertase subtilisin/kexin type 9 (PCSK9) is a serine protease enzyme encoded by the PCSK9 gene in humans and it plays a role in regulation of circulating cholesterol. It is associated with autosomal dominant hypercholesterolemia, a state of elevated levels of LDL (low-density lipoprotein) cholesterol. Autosomal dominant hypercholesterolemia can result in severe implications such as stroke and coronary heart disease. |
Alternative Name | NARC1; NARC-1; PC9; Proprotein convertase subtilisin/kexin type; Neural apoptosis-regulated convertase 1; Proprotein convertase 9; Subtilisin/kexin-like protease PC9; PA4Fab |
UniProt | Q8NBP7 |
Immunogen | The original antibody was isolated from a natural human Fab phage display library by panning against recombinant human PCSK9. Later one the Fab antibody was converted to IgG1 format. |
Application Notes | The human IgG1 format of this antibody was reported to bind human PCSK9 with a binding affinity Kd= 1.05 nM. The binding characterization of this antibody to human PCSK9 was done using ELISA and western blot. In an in vitro competition binding ELISA, IgG1-PA4 blocked PCSK9 from binding to LDLR, with an IC50 of approximately 7 nM. In vitro, it also attenuated PCSK9-mediated degradation of LDLR on the HepG2 cell surface in a dose dependent manner. In C57BL/6 mice, administration of IgG1-PA4 at 30 mg/kg increased hepatic LDLR protein levels by as much as 3 fold when compared with control. This antibody has potential for being used as a therapeutic for the treatment of hypercholesterolemia by inhibiting PCSK9-mediated degradation of cell surface LDLRs (PMID: 26056005). |
Antibody First Published | Cao et al. Selection and characterization of human PCSK9 antibody from phage displayed antibody library. Biochem Biophys Res Commun. 2015 Aug 7;463(4):712-8. PMID:26056005 |
Note on publication | This paper describes the isolation of a Fab antibody that binds human PCSK9. The IgG1 format was generated and characterized. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
