Key features and details | |
Cat. No. | MABL-2551 |
Name | Anti-OspA mAbs |
Clone No. | AFD-184.1 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | crystallisation, WB, ELISA |
Species Reactivity | Borrelia burgdorferi |
Basic Information | |
Specificity | 184.1 was shown to react specifically with a common OspA epitope shared by a homologous protein of lower molecular weight (Jiang et al, 1990). Based on N-terminal sequencing and reactivity of 184.1 against truncated versions of OspA, it was concluded that 184.1 recognises an N-terminal epitope of OspA. This was corroborated by a crystal structure of the OspA-184.1 Fab structure (Li et al, 1997), which showed that 184.1 binds a well-ordered beta sheet arrangement near the N-terminus. |
Alternative Name | Outer surface protein A |
UniProt | P0A3N6 |
Immunogen | BALB/c mice were immunised by injection of B. burgdorferi sonicated preparations. Splenocytes were obtained from immunised mice and fused with X63-Ag8 myeloma cells to generate hybridomas. |
Application Notes | 184.1 was used in Western blots and ELISA to determine specificity against B. burgdorferi major surface proteins (Jiang et al, 1990). |
Antibody First Published | Jiang et al. Cross-antigenicity between the major surface proteins (ospA and ospB) and other proteins of Borrelia burgdorferi. J Immunol. 1990 Jan 1;144(1):284-9. PMID:2295795 |
Note on publication | Describes the generation and use of mAbs against B. burgdorferi to characterise structural heterogeneity between the major surface proteins OspA and OspB. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
