Key features and details | |
Cat. No. | MABL-2505 |
Name | Anti-Notch 1 mAbs |
Clone No. | AFD- N248A |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | Block, ELISA |
Species Reactivity | Human |
Basic Information | |
Specificity | This antibody binds to notch 1 at two distinct sets of epitopes, one in Lin-A domain and other in HD-C domain (aa 1705- 1710), causing the notch 1 to be locked down in the silent conformation, and thus preventing the receptor from being activated by its ligands. |
Alternative Name | Notch1; hN1; TAN-1; Neurogenic locus notch homolog protein 1; Translocation-associated notch protein TAN-1 |
UniProt | P46531 |
Immunogen | The original antibody was generated by immunizing BALB/c mice with recombinant notch 1 fusion protein as immunogen. The fusion protein contained N-terminal leader peptide, EGF-like repeats 35 to 36, NRR (including Lin A, B and C domains and the HD domain) and a small portion of intracellular sequence. |
Application Notes | This antagonist antibody binds notch 1 with an affinity of Kd= 0.33 nM. The binding epitopes of Notch 1 antibody was analyzed by domain swap and ELISA binding assays. mAb N248A inhibits both T-cell acute lymphoblastic leukemia (T-ALL) and breast cancer cell growth in cell culture. N248A also inhibits T-cell lymphoblastic leukemia in murine xenograft tumor model (WO2012080891). |
Antibody First Published | |
Note on publication | |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
