Key features and details | |
Cat. No. | MABL-2495 |
Name | Anti-non-structural protein 4 mAbs |
Clone No. | AFD- Nb43 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | in vitro, ELISA |
Species Reactivity | Porcine reproductive and respiratory syndrome virus |
Basic Information | |
Specificity | The antibody is specific for Nsp4. |
Alternative Name | NSP4; Replicase polyprotein 1ab; ORF1ab polyprotein |
UniProt | Q9YN02 |
Immunogen | An adult male Bactrian camel was immunized subcutaneously with the purified Nsp4-NHis recombinant protein. A camel VHH library was then constructed and panned against Nsp4. |
Application Notes | The specificity of the original format of the antibody was confirmed by indirect ELISA analysis. MARC-145 cell lines expressing nanobodies were generated and infected with PRRSV strain SD16. At 24 h post-infection the antibody inhibited infectious virus release by about 99%. Further, the antibody protected MARC-145 cells from any virus-induced cytopathic effect and fully blocked PRRSV replication (Liu et al. 2016; PMID: 27010387). |
Antibody First Published | Liu et al. Intracellularly expressed nanobodies against non-structural protein 4 of porcine reproductive and respiratory syndrome virus inhibit virus replication Biotechnol Lett. 2016 Jul;38(7):1081-8. PMID:27010387 |
Note on publication | The original paper describes the generation and characterization of a set of antibodies by phage display method. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
