Key features and details | |
Cat. No. | MABL-2458 |
Name | Anti-NAP1L1 mAbs |
Clone No. | AFD-Nb179 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | WB, ELISA |
Species Reactivity | Human |
Basic Information | |
Specificity | This antibody binds human nucleosome assembly protein 1-like 1 (NAP1L1). This protein is a histone chaperone that plays a role in the nuclear import of H2A-H2B and nucleosome assembly. It also participates in DNA repair mechanisms |
Alternative Name | NRP; hNRP; Nucleosome assembly protein 1; NAP-1-related protein |
UniProt | P55209 |
Immunogen | The original antibody was isolated from a VHH library generated by immunization of an alpaca with whole glioblastoma multiforme (GBM) cells. |
Application Notes | This antibody was used for identification of NAP1L1 protein in cytosolic and membrane protein fractions of glioblastoma (GBM) tissues using western blotting. It was reported that NAP1L1 was a good marker to distinguish glioma tissues from reference tissues. ELISA was used for the screening of the nanobody library and identification of specific binders that showed specificity for GBM proteins (PMID: 28498803). |
Antibody First Published | Jovčevska et al. Differentially expressed proteins in glioblastoma multiforme identified with a nanobody-based anti-proteome approach and confirmed by OncoFinder as possible tumor-class predictive biomarker candidates. Oncotarget. 2017 Jul 4;8(27):44141-44158. PMID:28498803 |
Note on publication | Describes the isolation of nanobodies against various antigens overexpressed in glioblastoma multiforme. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
