Key features and details | |
Cat. No. | MABL-2444 |
Name | Anti-myotoxic protein mAbs |
Clone No. | AFD-M85 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | neutralizing, ELISA |
Species Reactivity | Bothrops atrox |
Basic Information | |
Specificity | The original antibody is specific for the myotoxic protein of Bothrops atrox. |
Alternative Name | Myotoxin I; svPLA2 homolog; Phospholipase A2 homolog 1 |
UniProt | Q6JK69 |
Immunogen | The original antibody was generated by immunizing a two-year-old adult male L. glama with increasing doses of B. atrox venom, followed by a VHH library construction and panning against partially purified hemorrhagic and myotoxic proteins. |
Application Notes | The specificity of the original format of the antibody was confirmed by ELISA analysis. The original antibody was able to decrease myonecrosis induced by the whole venom, as evaluated by the CK activity levels in plasma (Calderon et al., 2020; PMID: 32457735). |
Antibody First Published | Calderon et al. Development of Nanobodies Against Hemorrhagic and Myotoxic Components of Bothrops atrox Snake Venom Front Immunol. 2020 May 7:11:655. doi: 10.3389/fimmu.2020.00655. eCollection 2020. PMID:32457735 |
Note on publication | The original paper describes the generation and characterization of the antibody. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
