Key features and details | |
Cat. No. | MABL-2367 |
Name | Anti-MHC I mAbs |
Clone No. | AFD- R1-21.2 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | IP, radioimmunoassay, WB, IHC |
Species Reactivity | Mouse |
Basic Information | |
Specificity | This antibody is specific for murine MHC class I, and binds to polymorphic H-2 determinants expressed on most haplotypes. This antibody is, therefore, able to bind all H-2 class I molecules, independent of the allele. This antibody inhibits the binding of most mouse anti-H-2 antibodies to H-2k cells. |
Alternative Name | R1.21.2; H-2; MHC-I; MHC Class I |
UniProt | |
Immunogen | This antibody was raised by immunising Lewis 1F rats with mouse CH-1 lymphoma cells. Spleen cells from immunised rats were fused with X-63Ag8-653 myeloma cells to generate hybridomas. |
Application Notes | This antibody binds murine H-2 MHC class I molecules, and has been used in both structural and biochemical studies. It has been employed to assess MHC class I expression in frozen, acetone-fixed mouse brain sections by immunohistochemistry (Miralvès et al, 2007), and to stain paraffin- embedded sections of mouse liver for H-2Kb expression (Limmer et al, 2005). It has also been used to precipitate mouse H-2Kk and H-2Dk class I molecules from detergent lysates of Ltk3.3/1 cells (Rada et al, 1990), and to stain H-2Kb and H-2Kk-expressing cells in FACS analysis (Bendelac, 1995). Cellular radioimmune assays have been used to confirm the binding of this antibody to spleen cells, and assess its strain specificity (Koch et al, 1983). |
Antibody First Published | Koch et al Comparison of allogeneic and xenogeneic determinants on the H- 2Kk molecule Transplantation, August 1983, Vol.36(2), pp.177-80 [Peer Reviewed Journal] PMID:6192569 |
Note on publication | Describes the original generation and characterisation of this antibody. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
