欢迎来到西安迈博睿(Mabioway)生物科技有限公司 Call: 15339069646 | E-mail: info@mabioway.cn 在线留言 |

服务热线

15339069646
网站导航
当前位置: 首页 > 产品展示 > 抗体产品 > 一抗 > 其他抗体
其他抗体

Anti-Melanotransferrin mAbs

简要描述:

The Fab fragment is specific for human melanotransferrin (MTf). In particular, it targets the C2 subdomain of the MTf C-lobe, making direct contact with ten MTf residues (Arg 458, Arg 459, Asp 460, Ser 461, His 463, Arg 474, Val 564, Glu 565, Asn 566, Asp 598) as well as two N-acetylglucosamines of the Asn 515 N-glycan. The Fab fragment cross reacts with cynomolgus monkey MTf but not with rat and mouse MTf. Melanotransferrin is a 75 kDa member of the transferrin superfamily, which comprises a single chain, iron- binding glycoproteins responsible for iron homeostasis in cells.

产品参数:

Cat. No.:MABL-2341

Species:Engineer

Species Reactivity:Human

Type:Recombinate

Application:crystallography, in vitro, in vivo, SPR, IHC

详细介绍

Key features and   details


Cat. No.

MABL-2341

Name

Anti-Melanotransferrin   mAbs

Clone No.

AFD- SC57.32

From

Recombinant Antibody

Isotype

Engineer antibody

Application

crystallography, in vitro, in vivo, SPR, IHC  

Species Reactivity

Human

Basic Information


Specificity

The Fab fragment is specific for human melanotransferrin   (MTf). In particular, it targets the C2 subdomain of the MTf C-lobe, making   direct contact with ten MTf residues (Arg 458, Arg 459, Asp 460, Ser 461, His   463, Arg 474, Val 564, Glu 565, Asn 566, Asp 598) as well as two   N-acetylglucosamines of the Asn 515 N-glycan. The Fab fragment cross reacts   with cynomolgus monkey MTf but not with rat and mouse MTf. Melanotransferrin   is a 75 kDa member of the transferrin superfamily, which comprises a single   chain, iron- binding glycoproteins responsible for iron homeostasis in cells.

Alternative Name

Melanoma-associated antigen p97; CD228; MTf;   MFI2

UniProt

P08582

Immunogen

The original antibody was generated by   immunizing Balb/c mice with human Melanotransferrin- His.

Application Notes

The crystal structure of the Fab fragment in   complex with human melanotransferrin (hMFt) was determined. Surface plasmon   resonance assay was performed to evaluate the binding specificity and   affinity between the Fab fragment and hMFt (Kd= 1.0 nM). The Fab fragment is   the murine version of SC-005, which has been used in clinical trials (Hayashi   et al., 2021; PMID: 33436675).Immunohistochemistry was performed on HEK293T   parental cell pellets or MFI2-expressing HEK293T cell pellets using the   murine version of the antibody, which was able to specifically detect   MFI2-overexpressing HEK293T cell pellets. The humanized version of the   antibody was able to kill HEK-293T cells overexpressing MFI2, showing comparable efficacy to the chimeric antibody. In vivo studies with the   humanized version of the antibody conjugated to a pyrrolobenzodiazepine resulted in significant tumor suppression in mice (US10428156).

Antibody First   Published

Hayashi et al. Complex of human   Melanotransferrin and SC57.32 Fab fragment reveals novel interdomain   arrangement with ferric N-lobe and open C-lobe. Sci Rep. 2021 Jan   12;11(1):566. PMID:33436675

Note on publication

The paper describes the structure of the Fab   fragment in complex with human melanotransferrin.

COA Information For reference only, actual COA shall prevail

Size

100 μg Purified   antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity   purified

Buffer

PBS with 0.02% Proclin   300.

Concentration

1 mg/ml.

Storage   Recommendation

Store at 4⁰C for up to 3   months. For longer storage, aliquot and store at - 20⁰C.


 


服务咨询

留言框

  • 标题:

  • 留言内容:

  • 您的单位:

  • 您的姓名:

  • 联系电话:

  • 常用邮箱:

  • 详细地址:

推荐产品

联系我们

Copyright © 2012-2029 西安迈博睿(Mabioway)生物科技有限公司 版权所有

地址:陕西省西安市浐灞生态区广运潭大道南段4555号长安大学科技园4号楼A单元2层

电话:15339069646 邮箱:info@mabioway.cn

陕ICP备2021006339号