Key features and details | |
Cat. No. | MABL-2328 |
Name | Anti-MARCO mAbs |
Clone No. | AFD- PLK1 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | flow cytometry, IP, WB, IF, IHC-Fr |
Species Reactivity | Human |
Basic Information | |
Specificity | This antibody recognises the extracellular domain V between residues 420 and 431 in MARCO both in human and bovine samples. |
Alternative Name | SCARA2; macrophage receptor MARCO; macrophage receptor with collagenous structure; scavenger receptor class A member 2; PLK-1; PLK 1 |
UniProt | Q9UEW3 |
Immunogen | The antibody was raised by immunising BALB/c mice with human alveolar macrophages (AMs) and subsequent creation of hybridomas. |
Application Notes | The specificity of this antibody was confirmed in an IF experiment where the antibody stained alveolar macrophages for MACRO (Arredouani et al., 2005). Tissue samples prepared in acetone and paraformaldehyde were also successfully immunohistochemically stained (Arredouani et al., 2005; Martens et al., 2006). The antibody was also capable of weak staining of alveolar macrophages in flow cytometry (Palecanda and Kobzik, 2001). In IP experiments, the antibody immunoprecipitates MARCO from lysates obtained from COS cells transfected with human MARCO (Arredouani et al., 2005). |
Antibody First Published | Arredouani et al. MARCO Is the Major Binding Receptor for Unopsonized Particles and Bacteria on Human Alveolar Macrophages J Immunol November 1, 2005, 175 (9) 6058-6064 PMID:16237101 |
Note on publication | The article describes the method of generation of anti-MARCO PKL1 antibody and its effectivenes in IHC, IP and functional experiments. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
