Anti-LAG-3 mAbs

Cat. No.

MABL-2220

Name

Anti-LAG-3 mAbs

Clone No.

AFD- ADI-31798

From

Recombinant Antibody

Isotype

Engineer antibody

Application

ELISA, FC

Species Reactivity

Human

Basic Information

Specificity

This antibody binds human LAG-3. The human   LAG-3 is a cell surface molecule with diverse biologic effects on T cell   function. It is an immune checkpoint receptor on activated T-cells.

Alternative Name

CD223; LAG3; FDC; Lymphocyte activation gene   3 protein

UniProt

P18627

Immunogen

The original antibody was isolated from a yeast   display library by two rounds of screening against human LAG-3.

Application Notes

This antibody can bind human LAG-3 in an   ELISA. This antibody binds human LAG-3 with an affinity of Kd= 9.73 X   10^(-10) nM. The binding specificity of this antibody to human LAG-3   expressed on the surface of HEK293 and activated CD4+ T cells was done using   flow cytometry. This antibody binds hLAG-3 expressed on the surface of HEK293   cells with an EC50 of 0.201 nM and activated CD4+ T cells with an EC50 of   0.00595 nM. This antibody can also effectively block the binding of LAG-3 and   its ligand MHCII (HLA-DR) with an IC50 of 4.701 nM. The tumor suppressive   effects of this antibody was studied in A375 human PBMC model (CN112166125).

Antibody First   Published

Note on publication

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified   antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity   purified

Buffer

PBS with 0.02% Proclin   300.

Concentration

1 mg/ml.

Storage Recommendation

Store at 4⁰C for up to 3   months. For longer storage, aliquot and store at - 20⁰C.