Anti-LAG-3 mAbs

Cat. No.

MABL-2219

Name

Anti-LAG-3 mAbs

Clone No.

AFD-AbAbLAG3

From

Recombinant Antibody

Isotype

Engineer antibody

Application

agonist, functional   assays, WB, ELISA

Species Reactivity

Human

Basic Information

Specificity

This antibody is   specific for LAG-3. LAG-3 is an immune checkpoint receptor found primarily on   the surface of T cells, including regulatory T cells and exhausted T cells.   It plays a critical role in downregulating immune responses. LAG-3 is  structurally similar to the well-known CTLA-4 and PD-1 checkpoint receptors   and binds to MHC Class II molecules, transmitting inhibitory signals to the T   cells. This dampens the immune response, helping to maintain immune   homeostasis and prevent overactivation, which could lead to tissue damage or   autoimmune diseases.

Alternative Name

Lymphocyte   Activation Gene 3; Lymphocyte activation gene 3 protein; CD223

UniProt

P18627

Immunogen

The original antibody   was selected from a camel-derived nanobody phage display library by screening   against biotinylated LAG-3.

Application Notes

The specificity of the   original antibody (camel VHH) was verified via ELISA and WB. In an in   vitro T cell and Daudi cells co-culturing experiment, the addition of   200 μg/ml of a human IgG Fc fused version of this antibody led to the best   killing effect on tumor cells. Furthermore, in a follow-up experiment, the   antibody was shown to promote the killing of tumor cells by T/CD19 CAR-T   cells without distinguishing tumor cell types when tested on K562 and Daudi   cells (CN114437219A).

Antibody First   Published

Note on publication

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified   antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity   purified

Buffer

PBS with 0.02% Proclin   300.

Concentration

1 mg/ml.

Storage   Recommendation

Store at 4⁰C for up to 3   months. For longer storage, aliquot and store at - 20⁰C.