Anti-Ku protein mAbs

Cat. No.

MABL-2208

Name

Anti-Ku protein mAbs

Clone No.

AFD- INCA-X

From

Recombinant Antibody

Isotype

Engineer antibody

Application

IP, RIA, FC

Species Reactivity

Human

Basic Information

Specificity

This antibody recognizes and binds the Ku   protein heterodimer (Ku70/80), a nuclear protein that is also present on the   surface of some tumor cells.

Alternative Name

Ku70; Ku 80; Ku70/80; XRCC5; XRCC6; Ku   heterodimer; X-ray repair cross-complementing protein 5; X-ray repair   cross-complementing protein 6; 70 kDa subunit of Ku antigen;   5'-deoxyribose-5-phosphate lyase Ku70; 5'-dRP lyase Ku70;ATP-dependent DNA   helicase 2 subunit 1; ATP- dependent DNA helicase II 70 kDa subunit; CTC   box-binding factor 75 kDa subunit; CTC75; CTCBF; Lupus Ku autoantigen protein   p70; Thyroid-lupus autoantigen; TLAA; 86 kDa subunit of Ku antigen; ATP-dependent   DNA helicase 2 subunit 2; ATP-dependent DNA helicase II 80 kDa subunit; CTC   box-binding factor 85 kDa subunit; CTC85; Lupus Ku autoantigen protein p86;   Nuclear factor IV

UniProt

P12956, P13010

Immunogen

The original antibody was isolated from a naïve   phage antibody library n-CoDeR®. First substraction was done by incubation   with the T cell leukemia cell line MOLT-4. Selections were performed on the   pancreatic adenocarcinoma cell line PL45, during 1 h incubation at 37° C,   allowing internalization of the phage scFv-receptor complexes. Internalized   phages were retrieved by lysing surface-stripped cells with 100 mM   triethylamine.

Application Notes

Ku protein is a heterodimer of 2 tightly   associated sub-units called the Ku70 and Ku80, normally located in the   nucleus, where it is involved in nonhomologous end-joining processes, which   is responsible for repairing double stranded DNA breaks. The   receptor-mediated endocytosis of Ku70/80 is rapid (t1/2 12 min) and extensive   (90% of the receptor pool inside the cell after 100 min) as measured by   rotating radioimmunoassay. Ku70/80 was also successfully used as a port of   entry for cytotoxic payloads to tumor cells of various origin. INCA-X   recognizes and binds Ku protein heterodimer. The binding specificity of   INCA-X for Ku70/80 was confirmed with immunoprecipitation and flow cytometry.   This antibody is capable of internalization and has the potential for use in   immunotherapy. The internalization of INCA-X in pancreatic carcinoma cells   was confirmed using confocal microscopy. 

Antibody First   Published

Fransson et al. The nuclear DNA repair   protein Ku70/80 is a tumor- associated antigen displaying rapid receptor   mediated endocytosis. Int. J. Cancer (2006): 119 (10), 2492–2496.   PMID:16929494

Note on publication

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified   antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity   purified

Buffer

PBS with 0.02% Proclin   300.

Concentration

1 mg/ml.

Storage   Recommendation

Store at 4⁰C for up to 3   months. For longer storage, aliquot and store at - 20⁰C.