Anti-KRAS G12V mAbs

Cat. No.

MABL-2206

Name

Anti-KRAS G12V mAbs

Clone No.

AFD- G12V-34

From

Recombinant Antibody

Isotype

Engineer antibody

Application

MTT cytotoxicity assay, ELISA, IF

Species Reactivity

Human

Basic Information

Specificity

This antibody is specific for mutated KRAS G12V.

Alternative Name

Mutated KRAS G12V; Mutated Kirsten rat   sarcoma viral oncogene homolog G12V; Kirsten Rat Sarcoma virus; Kirsten Rat   Sarcoma virus; C-K-RAS; CFC2; K-RAS2A; K-RAS2B; K- RAS4A; K-RAS4B; KI-RAS;   KRAS1; KRAS2; NS; NS3; RALD; RASK2; K-ras; KRAS proto-oncogene; GTPase;   c-Ki-ras2; OES; c-Ki-ras; K-Ras 2; 'C-K-RAS; K-Ras

UniProt

Immunogen

The original antibody was generated through   the construction of a phage-displayed single- chain fragment variable (scFv) library from mice splenocytes immunized with specific mimotope peptides. The   library was subjected to three rounds of biopanning on G12V and G13D   peptide-coated plates.

Application Notes

The original format of this antibody (scFv) was screened against WT and mutated G12 and G13 KRAS and shown to   specifically bind mKRAS G12V in an ELISA. The scFv format of this antibody was fused to mHALT-1 toxin and exhibited cytotoxic effects on SW-480 colorectal cancer cells with IC50 of 25.39 μg/mL, with minimal cytotoxicity   effect on NHDF cells. The scFv format of this antibody was fused to enhanced   GFP and used for IF (Teo et al., 2021; PMID: 33959410).

Antibody First   Published

Teo et al. Development of a single-chain   fragment variable fused-mutant HALT-1 recombinant immunotoxin against G12V   mutated KRAS colorectal cancer cells PeerJ. 2021 Apr 15:9:e11063. doi:   10.7717/peerj.11063 PMID:33959410

Note on publication

The original publication describes the   development of a targeted therapy for KRAS- mutated colorectal cancer cells   by creating a recombinant anti-mKRAS single-chain fragment variable fused   with mutant Hydra actinoporin-like-toxin-1 immunotoxin.

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified   antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity   purified

Buffer

PBS with 0.02% Proclin   300.

Concentration

1 mg/ml.

Storage Recommendation

Store at 4⁰C for up to 3   months. For longer storage, aliquot and store at - 20⁰C.