Key features and details | |
Cat. No. | MABL-2154 |
Name | Anti-Integrin alpha-1 mAbs |
Clone No. | AFD- AQC2 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | crystallography, in vivo, WB, ELISA |
Species Reactivity | Sheep, Human |
Basic Information | |
Specificity | This antibody is specific for VLA-1 |
Alternative Name | hAQC2; huAQC2; VLA-1; CD49a; ITGA1; Laminin and collagen receptor; CD49 antigen-like family member A |
UniProt | P56199 |
Immunogen | The original antibody was raised by immunizing a mouse with human VLA-1. |
Application Notes | The structure of the humanized version of this antibody was determined using x-ray crystallography. The humanized antibody was used for an ELISA on human alpha 1 beta 1 I domain (Karpusas et al., 2003; PMID:12662928). This antibody was used for a western blot on alpha-1 integrin of different animals, showing that this antibody could also bind sheep alpha-1 integrin. This antibody was used as an aerosol to prevent allergic reactions in the lungs of sheep. It only showed an effect on the late airway responses and no effect on the initial allergic reaction (Abraham et al., 2004; PMID:14578216). |
Antibody First Published | Gotwals et al. Divalent Cations Stabilize the alpha-1 Beta-1 Integrin I Domain Biochemistry. 1999 Jun 29;38(26):8280-8. PMID:10387073 |
Note on publication | The original paper generated and characterized a panel of antibodies directed against the alpha-1 I domain. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
